Preparation time, mini spin columns, manual or automated processing, for isolation of viral rna and dna and bacterial. The chemagic viral dnarna kit was evaluated for extraction of cytomegalovirus cmv, hepatitis b virus hbv, and hepatitis g virus hgv by using the qiaamp dna blood mini kit and the qiaamp viral rna mini kit as reference protocols. Qiaquick gel extraction kit protocol using a microcentrifuge. For purification of genomic and mitochondrial dna from small samples. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for pcr. Qiaampviralrnaminihandbook 122014 5 intendeduse the qiaamp viral rna mini kit is intended for molecular biology applications. No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic, prognostic, therapeutic, or blood banking. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed cells from urine. Qiaamp cador pathogen mini kit 250 from qiagen selectscience. Qiaamp dna mini kit and qiaamp dna blood mini kit 022003 7 product use limitations qiaamp kits are intended as generalpurpose devices. The purelink viral rnadna mini kit provides a rapid and efficient method to simultaneously purify viral rnadna from fresh or frozen cellfree biological fluids plasma, serum, cerebrospinal fluid and cell culture supernatants.
Spincolumn protocol 25 purification of total dna from animal tissues spincolumn protocol 28. For isolation of genomic, mitochondrial, bacterial, parasite or viral dna. Place the qiaamp mini columns into the vacconnectors on the. Qiaamp dna blood mini kits, or qiagen products in general, please do. Lysis with qiagen protease or proteinasek qiaamp dna blood. Qiagen qiaamp dna mini kit, 50 preps, 200l sample, 50 to 200l elution volume, whole blood, tissue, cells sample, silica technology, spin column format, manual processing, 20 min. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. Dna extraction protocol for iswabdna using qiaamp dna mini kit. Purelink viral rnadna mini kit thermo fisher scientific. Dna isolation will be performed using the qiaamp dna mini kit from qiagen cat no. Qiaamp viral rna mini handbook 122007 5 product use limitations the qiaamp viral rna mini kit is intended for general laboratory use. View qiaamp dna mini kit gdna extraction kit manual. Up to 400 mg agarose can be processed per spin column.
The qiaamp dna mini kit can be used to isolate dna from human tissues, swabs, blood or washed cells from urine. When using the qiaamp dna blood mini kit for a sample that. Laboratory protocol for dna extraction from oracollectdx ocd. Intensive research has shown that qiagen protease is the optimal enzyme for use with the lysis buffer provided in the qiaamp dna blood mini kit. Supp prot saliva dna qiagen qiaamp blood mini kit v1. Processing nondsp qiaamp mini and qiaamp minelute spin columns on the qiavac 24 plus a vacuum pump capable of producing a vacuum of at least 900 mbar e. We recommend all users of qiagen products to adhere to the nih guidelines. Efficient lysis with no mechanical homogenization only 20 minutes of total handson preparation time purification of highquality dna from a wide range of samples the qiaamp dna mini kit can be used to isolate dna from human tissues, swabs, blood or washed cells from urine. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Scope and purpose qiagen qiaamp kits provide a fast, easy, and reliable method to purify total dna from small quantities of human tissue or blood. Qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation. When you sample feces, its better to take the one of the extremities as it contains more cells. These are available online in convenient and compact pdf format.
Dna extraction protocol for iswabdna using qiaamp dna mini. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for pcr, southern. The qiaamp dna micro kit simplifies purification of genomic and mitochondrial dna with a fast spincolumn procedure. The purelink viral mini kit is specifically designed to isolate highq. Qiaamp dna micro kit 50 56304 from qiagen sample to. Orders 80057296 fax 80075951 technical 800dnaprep 8003627737 france qiagen s. Dna is extracted from peripheral blood using qiaamp dna mini kit qiagen in accordance to the blood and body fluid protocol of the user manual supplied with the kit. Variations in the composition of the gut microbiota between individuals are increasingly recognized due to the development of cultureindependent analytic techniques smith et al. Qiagen qiaamp dna blood mini kit, 50 preps, 1 to 200l sample, 50 to 200l elution volume, whole blood, body fluids sample, spin column format, silica technology, manual processing, 20 to 40 min. Qiagen provides a variety of qiaamp genomic dna kits for fast, efficient. Qiagen qiaamp dna mini kit protocol qiagen dna qiagen dna blood mini kit protocol qiaamp dna blood midi maxi.
Qiaamp mini and qiaamp minelute spin columns are processed on the. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 100 l. Efficient extraction of viral dna and viral rna by the. No mechanical homogenization is necessary as the tissues are lysed enzymatically. Zymopure plasmid isolation kits provide the fastest and. Qiaamp cador pathogen mini handbook 122014 7 introduction the qiaamp cador pathogen mini kit enables the efficient purification of viral rna and dna and bacterial dna from a broad range of animal samples including whole blood, serum, plasma, swabs, washes, and tissue see starting material on page 14. Qiagen supplementary protocol purification of map dna from tissue using the qiaamp cador pathogen mini kit this protocol is designed for purification of spp. A comparison of the efficiency of five different commercial.
Qiagen protease is completely free of dnase and rnase activity. Preparation time, mini spin columns, manual or automated processing, for isolation of viral rna and dna and bacterial dna from a variety of. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. In this study, a semiautomatic dna extraction system easymag, biomerieux, marcy ietoile, france and a manual one qiaamp dna stool mini kit, qiagen, hilden, germany were tested on stool samples collected from 3 patients with inflammatory bowel disease ibd and 5 healthy individuals. Third edition june 2012 qiaamp dna mini and blood mini handbook for dna purification from. The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications.
Dna adsorbs to the dneasy membrane in the presence of high concentrations. Qiaamp dna mini and blood mini handbook 112007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Qiagen qiaamp dna micro kit, 50 preps, 1 to 100l sample, 20 to 100l elution volume, whole blood sample, silica technology, manual processing, spin column format, genomic dna, mitochondrial dna purification, 30 min. This kit can also be used for dna cleanup from enzymatic reactions see page 8. Do not add bleach or acidic solutions directly to the samplepreparation. Five widely used commercial deoxyribonucleic acid dna extraction kits qiasymphony virusbacteria midi kit kit qs, zr fecal dna miniprep kit z, qiaamp dna stool mini kit kit qa, ultraclean fecal dna isolation kit kit u and powersoil dna isolation kit kit p were evaluated, using human faecal samples. Qiaamp dna micro handbook 082003 5 product use limitations qiaamp kits are intended as generalpurpose devices. Standard operating procedures clinical protocol v dna isolation. The convenient spincolumn procedure reduces handson preparation time to 20 minutes. Rneasy mini protocol for isolation of total rna from heart, muscle. Briefly, bacterial samples were homogenized in buffer asl and heated at 95 c for 5 min to lyse bacterial cells. These products are not intended for the diagnosis, prevention, or treatment of a disease. Microbial diversity in fecal samples depends on dna.
Introduction th e oragenedna selfcollection kit allows saliva. Evaluation of qiaamp dna stool mini kit for ecological. Dneasy plant handbook 102012 7 introduction dneasy plant kits provide a fast and easy way to purify dna from plant and fungal tissue. Dna purification from dried blood spots qiaamp dna mini kit. For purification of up to 12 g genomic, mitochondrial, or viral dna from blood and related body fluids. The qiaamp dna mini kit yields dna sized up to 50 kb see figure purification of up to 50 kb genomic dna. Qiagen qiaamp dna mini kit, 250 preps, 200l sample, 50 to 200l elution volume, whole blood, tissue, cells sample, silica technology, spin column format, manual processing, 20 min. Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. Laboratory protocol for dna extraction from oracollectdx. Qiaamp dna mini kit and qiaamp dna blood mini kit 092001 7 product use limitations qiaamp kits are intended as generalpurpose devices. Standard operating procedure sop for dna extraction with qiaamp mini kit i. Extraction of dna from blood samples using qiaamp dna mini. The following risk and safety phrases apply to components of the rneasy mini kit, the rneasy protect mini kit, andor the rneasy plant mini kit. Up to 100 mg of tissue can be processed using the dneasy plant mini kit or up to 1 g of tissue using the dneasy plant maxi kit.
Lyophilized qiagen protease can be stored at room temperature 1525c for up to 6 months without any decrease in performance. Dna purified using the qiaamp dna mini kit can be used in a wide range of downstream applications, including pcr and quantitative realtime pcr, southern blotting, snp and str genotyping, and pharmocogenomic research. All due care and attention should be exercised in the handling of the products. Lysis with qiagen protease or proteinasek qiaamp dna blood mini kits contain qiagen protease. Qiaamp dna blood mini kit 50 from qiagen selectscience. Midi kit up to 1 ml for known biomarkers with high abundance. Qiagen qiaamp cador pathogen mini kit, 250 preps, 50 to 150. Extraction of dna from blood samples using qiaamp dna mini kit. Qiagen provides a variety of qiaamp genomic dna kits for fast. The qiaamp viral rna mini kit simplifies purification of viral rna from cellfree body fluids with fast spincolumn or vacuum procedures.
Plasma dna extraction using qiaamp circulating nucleic acid kit. Standard operating procedure sop for dna extraction with. The extraction efficiencies of the different kits for cmv dna and hbv dna were not distinguishable, but the extraction efficiency. Viral rna binds specifically to the qiaamp silica membrane, and pure viral rna is eluted in either water or a buffer provided with the kit. Do not add qiagen protease or proteinase k directly to buffer al. Special protocols for yeast and bacteria are included in the kit handbook. For isolation of viral rna and dna and bacterial dna from a variety of animal sample types. This protocol is designed for purification of total dna from grampositive bacteria. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. Qiaamp dna micro handbook 082003 7 introduction the qiaamp dna micro kit uses wellestablished technology for purification of genomic and mitochondrial dna from small sample volumes or sizes. The purified dna is up to 50 kb in size, with fragments of 20 30 kb predominating. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. Qiaamp dna stool mini kit was purchased from qiagen canada and used according to the manufacturers instructions.